In-Person Gas Chromatography: Fundamentals, Troubleshooting, and Method Development

Gain practical laboratory experience in gas chromatography. The course provides the fundamentals to understand the techniques and instrumentation involved in this powerful analytical tool.

Pricing: Starting USD 2995
Duration: >1 day
Coming Soon

About the Instructor(s)

Lee N. Polite
 President,  Axion Analytical Labs, Inc

Dr. Lee N. Polite is the president and founder of Axion Analytical Labs, Inc. He has taught chromatography to customers from every major pharmaceutical, chemical, and petroleum company in the U.S.

About the Course

Youtube ID: AlffEd05FoA

This course is designed for beginners and intermediate-level practitioners who want practical laboratory experience in gas chromatography (GC).

The lectures—supplemented by problem sets, slides, and video presentations—provide the fundamentals needed to understand the technique and instrumentation involved in this powerful analytical tool. In this class, you will master the fundamentals of GC, participate in five hands-on laboratory sessions, perform reference and literature searches, and learn specialized techniques based on your specific interests.

  • Overview of Gas Chromatography
  • Injection Techniques
  • Quantitative Analysis
  • Columns and Detectors
  • Temperature Programming
  • Method Development

What You Will Learn

  • Master the fundamentals of GC
  • Participate in five hands-on laboratory sessions
  • Perform reference and literature searches
  • Learn specialized techniques based on your specific interests

Who Should Attend

Beginners and intermediate-level practitioners in gas chromatography who want practical laboratory experience.

No previous experience in gas chromatography is required for the course.

Course Outline

  • Day 1: Gas Chromatography (GC) Overview & Injection Techniques

    Introduction to GC and instrumentation
    The GC Separation:  How it occurs
    Packed & Capillary columns
    Capillary inlet systems: split, splitless, on-column
    Laboratory Experiments:
    GC Familiarization / Temperature effects
    Split ratio
    Comparing split and splitless injection techniques

    Laboratory Experiments:

    GC Familiarization / Temperature effects
    Split ratio
    Comparing split and splitless injection techniques


  • Day 2: Resolution, Columns and Quantitative Analysis

    Resolution Equation (how to choose the correct chromatographic conditions)
    GC Theory Overview – Explains why we like small diameters and thin films
    Capillary column technology; column dimension and stationary phase selection
    Calibration methods: Area % – internal and external standard

    Laboratory Experiments:

    GC Parameters
    Detailed investigation of temperature and flow effects on a separation

  • Day 3: Detectors, GC/MS, Headspace

    Overview of available detectors; details of FID & TCD
    GC/MS – How it works and why it is important
    Headspace GC – Theory, practice and instrumentation

    Laboratory Experiments:

    Column installation
    Injector disassembly and maintenance
    Quantitation of an unknown employing internal vs. external standard techniques

  • Day 4:  Method Development & Troubleshooting/Maintenance

    Temperature programming
    Fundamentals of method development
    Troubleshooting Fundamentals
    Troubleshooting from actual chromatograms

    Laboratory Experiments:

    Develop a method for a completely unknown mixture.
    Optimize the newly developed method for speed.
    Participants will end up with a method that runs in less than 2 minutes…for a baseline separation of 11 compounds!

Dates, Locations, and Prices

Course fee includes tuition, lecture and laboratory materials, morning and afternoon snacks, and all lunches.

View complete details about lodging, local dining options, and available transportation here.

The course runs from 9:00 AM to 5:30 PM each day, class ends at 3:00 on Friday

Check in opens at 9:00 AM on the first day of the course.


Axion Analytical Laboratories
14 N Peoria Street, Suite 100
Chicago, IL 60607

Early Bird Full Price
Member(Premium Package) $2995
Member or Associate(Standard or Basic Package) $3195
Non-Member $3195

Course date and location to be posted. Please check back later for schedule update.