On-Demand High Performance Liquid Chromatography Basics
Gain a sound understanding of the basic principles of high-performance liquid chromatography.
About the Instructor(s)
Dennis Anderson retired from Akzo Nobel Surfactants America following a 40+-year career as an industrial analytical chemist.
About the Course
This course will help you gain a sound working knowledge of basic, high-performance liquid chromatographic principles and develop a greater understanding of theoretical and practical relationships.
Build the expertise you need to choose optimized column parameters, detector selection, and separation mechanism for the examination of complex mixtures, and improve your on-the-job performance.
- HPLC theory and best practices
- Column optimization
- Detector selection
- Improve separation of complex mixtures
What You Will Learn
- Move beyond standard operating procedures, and gain a solid working knowledge of HPLC principles and methods.
- Build expertise that will help you choose the right column, conditions, detector, and separation mechanism to separate difficult mixtures.
- Improve your on-the-job performance and skill set.
Who Should Attend
Ideal for R&D scientists and technicians or anyone who uses HPLC and wishes to have a solid theoretical and practical background in this technique.
Module 1 – Introduction
- Introduction to HPLC
- Separation Mechanisms
- Components of HPLC Instrumentation
- Commercial HPLC Instruments
Module 2 – Theory of HPLC
- Selection of Separation Mechanism
- Features of HPLC Chromatograms
- Resolution of HPLC Columns
- Resolution vs. Selectivity
Module 3 – Instrumentation
- Mass Spectrometry
- Refractive Index
- Evaporative Light Scattering
Module 4 – Separation Mechanisms
- Normal Phase
- Reverse Phase
- Anionic and Cationic Ion Exchange
- Steric Exclusion
Module 5 – Qualitative Analysis
- Retention Time
- Retention Indices
- Ultraviolet Spectroscopy
- Infrared Spectroscopy
- Mass Spectroscopy
Module 6 – Quantitative Analysis
- Integration Methods
- Area Percent
- Use of Response Factors
- Internal Standard
- External Calibration
- Method of Additions
Module 7 – Alternate and Optimization Techniques
- Enhanced UV Detection
- Capillary Zone Electrophoresis
- Octanol / Water Partition Coefficients
- Gradient Optimization
- Methods Development Criteria
- Computer Based Optimization
Dates, Locations, and Prices
On Demand course modules can be purchased a la carte for $199, each. All modules are held on a rolling basis throughout the year to accommodate your schedule and budget.
|Early Bird||Full Price|
|Member or Associate(Standard or Basic Package)||$199|