Online Live HPLC and UHPLC for Practicing Scientists 2: Best Practices in Method Development and Operation/Troubleshooting

Intermediate-level course on HPLC and UHPLC method development, operation, maintenance, troubleshooting, and UHPLC best practices.

Pricing: Starting USD 895
Duration: 3–8 hours
Registration Info

About the Instructor(s)

Michael W. Dong
Principal Consultant, MWD Consulting

Dr. Michael W. Dong is a principal consultant at MWD Consulting, focusing on consulting and training services on HPLC/UHPLC, pharmaceutical analysis, and drug quality. 

About the Course

This intermediate one-day workshop will provide the analytical scientist with a clearer understanding and solid working knowledge of best practices (method development, operation, and troubleshooting) of HPLC and UHPLC (ultra-high-pressure liquid chromatograph). The focus is on pharmaceutical analysis of small molecule drugs. A brief survey of biopharmaceutical, food, environmental, chemical, and bioscience applications is included. 

  • HPLC Applications and an Overview of UHPLC
  • HPLC Method Development and Validation
  • HPLC Operation and Troubleshooting 

Recommended textbook: M. W. Dong, HPLC and UHPLC for Practicing Scientists, 2nd Ed., Wiley, Hoboken, NJ, 2019

What You Will Learn

  • Concepts, perspectives, best practices, and potential issues of UHPLC
  • Fundamentals of HPLC method development and easier approaches
  • Overview of method validation and transfer
  • Standard operating procedures for HPLC modules, and troubleshooting strategies

Who Should Attend

Analysts, scientists, researchers, and managers in pharmaceutical and other industries who want to get an updated overview of the best practices of HPLC and UHPLC in pharmaceutical analysis including HPLC method development, operation, and troubleshooting. A basic understanding of chemistry and HPLC with some hands-on experience is assumed. 

Course Outline

  • HPLC Applications and an Overview of UHPLC

    • Overview and perspectives, UHPLC concepts (the need for high pressure, void volume, peak volume, instrumental bandwidth), UV flow cell design, dwell volume.
      Benefits and case studies: very fast separations with good resolution, very high-resolution analysis of complex samples, facilitating rapid method development, flexibility to customize resolution
    • Potential issues and how to mitigate (viscous heating, operating nuances, compatibility to existing methods, injection precision, detector sensitivity vs. mixing volumes), method translation from HPLC to UHPLC and vice versa using geometric scaling, how to transition from HPLC to UHPLC, end-fittings
    • HPLC applications including pharmaceutical, biopharmaceuticals, food, environmental, chemical, and bioseparations
    • UHPLC overview, perspectives, and benefits
    • Potential issues and how to mitigate  
  • HPLC Method Development and Validation

    • Tradition strategy and a survey of automated tools
    • 3-pronged template approach 
    • Use of a universal generic gradient method 
    • Method validation and method transfer
  • HPLC Operation and Troubleshooting

    • Mobile phase and sample preparation and best practice in HPLC operation
    • Problem diagnosis and troubleshooting guide with case studies
    • Diagnosing and solving problems (pressure, baseline, peak, data performance)
    • Mobile phase and sample preparation and HPLC operation  
    • Maintenance and troubleshooting guide
    • Diagnosing and solving problems 


Dates, Locations, and Prices

Five for four! Register five people for one course, one person for five courses, or any combination in between and your fifth registration is free. The free registration will be the course of the lowest price. Please note: This discount cannot be combined with any other discount offered. 


Online Live

Oct 05 - Oct 07, 2022

(10/5, 10/6 and 10/7)

Early Bird Full Price
Member(Premium Package) $895
Member or Associate(Standard or Basic Package) $1095
Non-Member $1095

This course will meet online October 5-7 from 11:00 AM to 1:30 PM ET. Course fee includes electronic access to the course materials and session recordings. Each person attending must register individually for this course.