In-Person Practical LC-MS/MS Method Development

This is a practical course for the practicing analytical chemists. After this course, the participants will be able to independently develop their own LC-MS/MS methods to use in their laboratories.

Pricing: Starting USD 895
Duration: 3–8 hours
Registration Info

About the Instructor(s)

Picture of Perry Wang
Perry G. Wang
Chemist, FDA

Dr. Perry G. Wang worked in the pharmaceutical and medical-device industry from 2000 to 2008. He received his Ph.D. at Oregon State University. Dr. Wang currently works at FDA as a chemist. His expertise in the pharmaceutical field focuses on high throughput drug analysis and validation by LC-MS.

About the Course

This LC-MS/MS short course is designed to offer practical training for the practicing scientists in the analytical field. This course will provide the participants with an updated overview and a solid working knowledge of LC-MS/MS. It will take the participants step-by-step through the concepts and techniques to develop LC-MS/MS methods. The emphasis is on practical issues associated with LC-MS/MS method development. It also focuses on problem-solving skills with examples encountered in the analytical fields. The participants will learn useful theoretical concepts, practical fundamentals and operating principles. After this course, the participants will be able to independently develop their own LC-MS/MS methods to use in their laboratories.

New technologies and techniques, such as hydrophilic interaction liquid chromatography (HILIC), monolithic chromatography, and core-shell particles for HPLC will be presented.

  • How column physical and chemical properties affect the resolution
  • How pH affects the separation and how to optimize a gradient profile
  • How to transfer HPLC methods to UHPLC/UPLC methods
  • How to select MS ionization modes
  • How to eliminate and compensate for matrix effects on MS
  • How to optimize and validate LC-MS/MS methods

What You Will Learn

  • The essence of the master resolution equation
  • How to deal with the challenges to separate polar molecules, e.g., proteins and peptides
  • What you need to know to develop a successful LC-MS/MS method
  • How to take advantage of the "orthogonality" of HPLC and MS

Who Should Attend

This one-day course is intended for analytical chemists, supervisors, lab managers, and researchers using LC-MS/MS. It will benefit the scientists ranging from college graduates to professionals in the analytical field.

Course Outline

  • 1. Introduction and Overview

    • History of chromatography
    • Introduction of high performance liquid chromatography
    • Introduction of mass spectrometry
  • 2. Key Concepts

    • Retention time (tR)
    • Retention factor (k’)
    • Separation factor (α)
    • Column efficiency (N)
    • Chromatographic resolution (R)
    • pKa/pKb of analytes
    • van Deemter Equation
    • Fundamentals of mass spectrometry
    • Atmospheric pressure ionization (API) in mass spectrometry
    • Common ionization modes: ESI, APCI and APPI
    • Mass analyzers: quadrupole, time of flight, ion trap and orbitrap
    • Mass resolution and mass accuracy
    • Matrix effects
  • 3. Operating Parameters and Column Selection

    • Flow rate and gradient time
    • Column temperature (T) & packed columns (support type, dimensions, particle & pore size)
    • Monolithic and HILIC columns
  • 4. Mass Spectrometer (MS)

    • Fundamental – charged species, mass resolution and mass accuracy
    • What kind of ionization should be selected – ESI, APPI or APCI
    • Single and Triple quadrupole, TOF, Ion trap and Q-exactive
    • How to develop an MS and MS-MS methods
    • How to perform an qualitative and quantitative analysis
  • 5. Method Development Approaches

    • Finding or estimating pKa or pKb of the analytes
    • Defining method type (reversed phase or normal phase or HILIC)
    • Estimating buffer pH
    • Scouting gradient to get the first chromatogram
    • Fine-tuning and optimizing the method – solvent type and strength

Dates, Locations, and Prices

In-Person

Mar 26

Indianapolis, IN

Early Bird Full Price
Member(Premium Package) $895 Ends Feb 26 $1095
Member or Associate(Standard or Basic Package) $1095 Ends Feb 26 $1295
Non-Member $1095 Ends Feb 26 $1295

Course Times: 8:30 AM - 5:00 PM; Check-in starts at 7:45 AM on the first day of the course. Course fee includes a course manual and continental breakfast each day. *Early Bird pricing ends four (4) weeks before the start date of the course.